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ctc and dapi stains  (Millipore)


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  • 90

    Structured Review

    Millipore ctc and dapi stains
    Ctc And Dapi Stains, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ctc+dapi/dapi/pm28808937-90-7-9
    Average 90 stars, based on 1 article reviews
    ctc and dapi stains - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Individual Dopaminergic Neurons of Lamprey SNc/VTA Project to Both the Striatum and Optic Tectum but Restrict Co-release of Glutamate to Striatum Only.
    Article Snippet: Following 3x10min washes in TNT-buffer, sections were incubated with Cy3 conjugated goat anti-rabbit IgG (1:500; Jackson ImmunoResearch; RRID_AB_2338000), 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 200 mg/L) and for the combined tract tracing experiments Neurobiotin was visualized with streptavidin-Alexa 633 (1:300; Thermo Fisher; Cat #S21375) in TNB for 2 h. For vGluT mRNA FISH combined with TH immunohistochemistry (N = 4), bound riboprobes were visualized with peroxidase-conjugated anti-DIG antibody (1:300; Roche Diagnostics; RRID_AB_514500) and TH with the rabbit anti-TH antibody (AB152; Merck Millipore; 1:200; RRID_AB_390204) in TNB at 4 C overnight.

    Fluorescence In Situ Hybridization:

    Article Title: Individual Dopaminergic Neurons of Lamprey SNc/VTA Project to Both the Striatum and Optic Tectum but Restrict Co-release of Glutamate to Striatum Only.
    Article Snippet: Following 3x10min washes in TNT-buffer, sections were incubated with Cy3 conjugated goat anti-rabbit IgG (1:500; Jackson ImmunoResearch; RRID_AB_2338000), 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 200 mg/L) and for the combined tract tracing experiments Neurobiotin was visualized with streptavidin-Alexa 633 (1:300; Thermo Fisher; Cat #S21375) in TNB for 2 h. For vGluT mRNA FISH combined with TH immunohistochemistry (N = 4), bound riboprobes were visualized with peroxidase-conjugated anti-DIG antibody (1:300; Roche Diagnostics; RRID_AB_514500) and TH with the rabbit anti-TH antibody (AB152; Merck Millipore; 1:200; RRID_AB_390204) in TNB at 4 C overnight.

    Immunohistochemistry:

    Article Title: Individual Dopaminergic Neurons of Lamprey SNc/VTA Project to Both the Striatum and Optic Tectum but Restrict Co-release of Glutamate to Striatum Only.
    Article Snippet: Following 3x10min washes in TNT-buffer, sections were incubated with Cy3 conjugated goat anti-rabbit IgG (1:500; Jackson ImmunoResearch; RRID_AB_2338000), 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 200 mg/L) and for the combined tract tracing experiments Neurobiotin was visualized with streptavidin-Alexa 633 (1:300; Thermo Fisher; Cat #S21375) in TNB for 2 h. For vGluT mRNA FISH combined with TH immunohistochemistry (N = 4), bound riboprobes were visualized with peroxidase-conjugated anti-DIG antibody (1:300; Roche Diagnostics; RRID_AB_514500) and TH with the rabbit anti-TH antibody (AB152; Merck Millipore; 1:200; RRID_AB_390204) in TNB at 4 C overnight.



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